ADHP;10-Acetyl-3,7-dihydroxyphenoxazine 

1-(3,7-Dihydroxy-10H-phenoxazin-10-yl)ethanone; Amplisyn Red

Fluorogenic substrate for peroxidase.

10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) is a highly sensitive, stable substrate for horseradish peroxidase (HRP) that enables selective detection of H2O2. This colorless, non-fluorescent reagent reacts with H2O2 to produce the fluorescent compound resorufin, which can be analyzed using an excitation wavelength of 520-550 nm and an emission wavelength of 585-595 nm. In a 96-well plate format, ADHP enables detection of H2O2 at a concentration as low as 5 pmol per 100 µl sample.