148705-09-3 , 4-Nitrophenyl lacto-N-tetraoside

148705-09-3 , 4-Nitrophenyl lacto-N-tetraoside ;
LNT-pNP; Gal-b-1,3-GlcNAc-b-1,3-Gal-b-1,4-Glc-b-pNP,
Cas:148705-09-3
C32H48N2O23 / 828.72

4-Nitrophenyl lacto-N-tetraoside

LNT-pNP; Gal-b-1,3-GlcNAc-b-1,3-Gal-b-1,4-Glc-b-pNP

4-Nitrophenyl lacto-N-tetraoside is a chromogenic substrate for enzymes that cleave lactose-specific linkages, such as beta-galactosidases. Upon enzyme cleavage, the pNP group is released, resulting in a yellow-colored solution that can be spectrophotometrically quantified at 405 nm. This substrate is commonly used in detecting lactose hydrolysis activity, such as in dairy industry for production optimization studies, or in microbiology for bacterial lactose fermentation assays. Its tetrasaccharide structure resembles that of lactose and makes it a specific substrate for lactose-utilizing enzymes. The properties of 4-nitrophenyl lacto-N-tetraoside make it a useful tool in enzyme kinetics studies and screening of new lactose-utilizing microorganisms.

CAS Number	148705-09-3
Product Name	Gal beta(1-3)GlcNAc beta(1-3)Gal beta(1-4)Glc-beta-pNP
IUPAC Name	N-[(2S,3S,4R,5S,6S)-2-[(2S,3S,4S,5S,6S)-2-[(2S,3S,4R,5S,6S)-4,5-dihydroxy-2-(hydroxymethyl)-6-(4-nitrophenoxy)oxan-3-yl]oxy-3,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-5-hydroxy-6-(hydroxymethyl)-4-[(2R,3S,4S,5R,6S)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-3-yl]acetamide
Molecular Formula	C32H48N2O23
Molecular Weight	828.727
InChI	InChI=1S/C32H48N2O23/c1-10(39)33-17-27(56-31-23(45)21(43)18(40)13(6-35)52-31)19(41)14(7-36)51-29(17)57-28-20(42)15(8-37)53-32(25(28)47)55-26-16(9-38)54-30(24(46)22(26)44)50-12-4-2-11(3-5-12)34(48)49/h2-5,13-32,35-38,40-47H,6-9H2,1H3,(H,33,39)/t13-,14-,15-,16-,17-,18-,19+,20-,21-,22+,23-,24-,25-,26+,27+,28-,29-,30+,31-,32-/m0/s1
InChI Key	UUSZRTFVRDAQGP-RMCKFCKISA-N
SMILES	CC(=O)NC1C(C(C(OC1OC2C(C(OC(C2O)OC3C(OC(C(C3O)O)OC4=CC=C(C=C4)[N+](=O)[O-])CO)CO)O)CO)O)OC5C(C(C(C(O5)CO)O)O)O
Synonyms	Gal beta(1-3)GlcNAc beta(1-3)Gal beta(1-4)Glc-beta-pNP