2-Nitrophenyl b-D-xylobioside

2-硝基苯基-β-D-木二糖苷,

2-Nitrophenyl b-D-xylobioside is a chromogenic substrate for the enzyme β-galactosidase. It is used in food testing, diagnostics, and environmental testing as an indicator of microbial contamination. 2-Nitrophenyl b-D-xylobioside is also used in fluorescence assay for the determination of β-galactosidase activity. In this assay, it is converted to a fluorescent product by β-galactosidase and can be detected by a fluorometer. 2NBPX has been shown to be non toxic and biodegradable, with good solubility at pH 7.0 and a reasonably low melting point. 2NBPX is commonly used as a ligand for affinity chromatography or as an enzyme substrate for quantitative analysis of β-galactosidase activity in biological samples.