3459-18-5, 4-Nitrophenyl-2-acetamido-2-deoxy-β-D-glucopyranoside, CAS: 3459-18-5
C14H18N2O8 / 342.30
Chromogenic substrate for N-acetylglucosaminidase.
4-Nitrophenyl-N-acetyl-β-D-glucosaminide is a chromogenic substrate for N-acetyl-β-glucosaminidase. Hydrolysis of 4-nitrophenyl-N-acetyl-β-D-glucosaminide by N-acetyl-β-glucosaminidase releases 4-nitrophenol, which can be quantified by colorimetric detection at 405 nm as a measure of N-acetyl-β-glucosaminidase activity. It has been used to quantify the activity of N-acetyl-β-D-glucosaminidase in human serum and urine in parenterally fed patients.
4-nitrophenyl N-acetyl-beta-D-glucosaminide is an N-acetyl-beta-D-glucosaminide in which the anomeric hydroxy hydrogen is replaced by a 4-nitrophenyl group. It has a role as a chromogenic compound. It is a C-nitro compound and a N-acetyl-beta-D-glucosaminide. It derives from a 4-nitrophenol.
|Appearance||White crystal powder||Complies|
|Solubility||Soluble in DMF, insoluble in ether||Complies|
|Identification||IR and HPLC||Complies|
|Melting point||206℃ ~ 210 °C||207℃ ~209 ℃|
( [α]23/D, c =0.3 inH2O)
|-18 ~ -20||-19.5|
|Any other impurity||Max. 0.5%||Complies|
|Heavy metal||Max. 20ppm||Complies|
|Residue on ignition||Max. 0.5%||0.10%|
|TLC||Should be one spot||One spot|
|Assayby HPLC||Min. 98%||99.20%|
|CAS No: 3459-18-5 Synonyms: GlcNAc-b-PNP4-Nitrophenyl N-acetyl-b-D-glucosaminePNP-GlcNAc4-Nitrophenyl N-acetyl-b-D-glucosaminide MDL No: MFCD00063696 Chemical Formula: C14H18N2O8 Molecular Weight: 342.30|
1. Muruhn D, Chim. Acta 1976, 73, 453
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