4-Methylumbelliferyl N,N-diacetyl-b-D-chitobioside

4-甲基伞形酮-beta-D-N,N’-二乙酰壳二糖苷

4-Methylumbelliferyl N,N-diacetyl-b-D-chitobioside is a fluorogenic substrate for peptide hormones. It is used in vitro to study the polymerase chain reaction (PCR) of human serum and other biological samples. 4-Methylumbelliferyl N,N-diacetyl-b-D-chitobioside is an orally bioavailable prodrug that undergoes hydrolysis by esterases to release 4-methylumbelliferone, which is then converted into the fluorescent product methylumbelliferone by cellular esterases. This product can be detected using a fluorescence sensor with high specificity and sensitivity. The fluorescence signal generated by this product is proportional to the enzyme concentration and can be used as a biomarker for peptide hormone production or neuronal death.

4-Methylumbelliferyl β-D-N,N'-diacetylchitobioside (4-ΜU-(GlcNAc)2) is a fluorogenic substrate for chitinases and chitobiosidases. 4-ΜU-(GlcNAc)2 is cleaved by chitinases and chitobiosidases to release the fluorescent moiety 4-ΜU. 4-ΜU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high pH (7.12-10.3), respectively, and an emission maximum ranging from 445 to 455 nm, increasing as pH decreases.