119171-73-2, ADHP;10-Acetyl-3,7-dihydroxyphenoxazine; 1-(3,7-Dihydroxy-10H-phenoxazin-10-yl)ethanone; Amplisyn Red

Cas:119171-73-2,
ADHP;10-Acetyl-3,7-dihydroxyphenoxazine; 1-(3,7-Dihydroxy-10H-phenoxazin-10-yl)ethanone; Amplisyn Red
C14H11NO4 / 257.24
MFCD00467861

ADHP;10-Acetyl-3,7-dihydroxyphenoxazine

1-(3,7-Dihydroxy-10H-phenoxazin-10-yl)ethanone; Amplisyn Red

Fluorogenic substrate for peroxidase.

10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) is a highly sensitive, stable substrate for horseradish peroxidase (HRP) that enables selective detection of H2O2. This colorless, non-fluorescent reagent reacts with H2O2 to produce the fluorescent compound resorufin, which can be analyzed using an excitation wavelength of 520-550 nm and an emission wavelength of 585-595 nm. In a 96-well plate format, ADHP enables detection of H2O2 at a concentration as low as 5 pmol per 100 µl sample.

CAS Number	119171-73-2
Product Name	10-Acetyl-3,7-dihydroxyphenoxazine
IUPAC Name	1-(3,7-dihydroxyphenoxazin-10-yl)ethanone
Molecular Formula	C14H11NO4
Molecular Weight	257.24 g/mol
InChI	InChI=1S/C14H11NO4/c1-8(16)15-11-4-2-9(17)6-13(11)19-14-7-10(18)3-5-12(14)15/h2-7,17-18H,1H3
InChI Key	PKYCWFICOKSIHZ-UHFFFAOYSA-N
SMILES	CC(=O)N1C2=C(C=C(C=C2)O)OC3=C1C=CC(=C3)O
Synonyms	10-acetyl-10H-phenoxazine-3,7-diol, 10-acetyl-3,7-dihydroxyphenoxazine, Amplex Red, amplex red reagent
Canonical SMILES	CC(=O)N1C2=C(C=C(C=C2)O)OC3=C1C=CC(=C3)O