3318-43-2 ,3-Indoxyl phosphate disodium salt

3318-43-2 ,3-Indoxyl phosphate disodium salt;
3-Indolyl phosphate disodium salt; 3-Indoxyl phosphate disodium salt
C8H6NNa2O4P / 257.09
MFCD00040646

3-Indoxyl phosphate disodium salt

3-Indolyl phosphate disodium salt; 3-Indoxyl phosphate disodium salt

Histochemical substrate for alkaline phosphatase.

3-Indoxyl phosphate is a chromogenic and electrochemical substrate for alkaline phosphatase. Upon enzymatic cleavage by alkaline phosphatase, 3-indoxyl is released and subsequently oxidized to produce water-insoluble indigo, which can be quantified by colorimetric detection at 660 nm as a measure of alkaline phosphatase activity. 3-Indoxyl phosphate can also be converted to water-soluble leucoindigo by reduction of indigo in alkaline solution by sodium dithionite, which can be quantified by colorimetric detection at 415 nm or voltametric detection as a measure of alkaline phosphatase activity. 3-Indoxyl phosphate has been used in ELISAs.

Indol-3-yl sodium phosphate is a white to off-white powder. (NTP, 1992)

CAS Number	3318-43-2
Product Name	Indol-3-yl sodium phosphate
IUPAC Name	disodium;1H-indol-3-yl phosphate
Molecular Formula	C8H6NNa2O4P
Molecular Weight	257.09 g/mol
InChI	InChI=1S/C8H8NO4P.2Na/c10-14(11,12)13-8-5-9-7-4-2-1-3-6(7)8;;/h1-5,9H,(H2,10,11,12);;/q;2*+1/p-2
InChI Key	WCSKWBKPKXJWEG-UHFFFAOYSA-L
SMILES	C1=CC=C2C(=C1)C(=CN2)OP(=O)([O-])[O-].[Na+].[Na+]
Solubility	greater than or equal to 100 mg/mL at 72° F (NTP, 1992)
Synonyms	3-Indolyl Phosphate Disodium Salt; 3-Indoxyl Phosphate Disodium Salt;
Canonical SMILES	C1=CC=C2C(=C1)C(=CN2)OP(=O)([O-])[O-].[Na+].[Na+]