4-Nitrophenyl phosphate bis(cyclohexylammonium) salt

4-Nitrophenyl phosphate bis(cyclohexylammonium) is an excellent chromogenic substrate designed for the detection and quantification of phosphatase enzyme activities. Aided by the presence of the cyclohexylammonium cation, it undergoes enzymatic hydrolysis, yielding the colored 4-nitrophenolate ion, which can be readily observed at 405 nm. The substrate has become an essential tool for biomedical research, playing a crucial role in unearthing cellular mechanisms, function, and potential drug targets.

Phosphoric acid, mono(4-nitrophenyl) ester, compd. with cyclohexanamine (1:2) is commonly known as Nitrophenylphosphoric acid. It is a synthetic compound that is used in various fields of research and industry. The compound is formed by the reaction of 4-nitrophenol with phosphorus pentoxide, followed by the reaction of the intermediate compound with cyclohexanamine. The compound is widely used in biological and chemical research due to its unique properties.

Physical and Chemical Properties:

Nitrophenylphosphoric acid is a fine, white crystalline powder. The compound is soluble in aqueous solutions, such as water or ethanol, but it is insoluble in most organic solvents. Nitrophenylphosphoric acid undergoes a hydrolysis reaction with water to form 4-nitrophenol and phosphoric acid.

The chemical formula for Nitrophenylphosphoric acid is C12H16N2O4P. The compound has a molecular weight of 288.24 grams per mole. The melting point of Nitrophenylphosphoric acid is 108-114°C, and the boiling point is 389.2°C.

Synthesis and Characterization:

The synthesis of Nitrophenylphosphoric acid involves two steps. First, 4-nitrophenol is reacted with phosphorus pentoxide to form an intermediate compound called nitrophenyl phosphorochloridate. Then, the intermediate compound reacts with cyclohexanamine to form Nitrophenylphosphoric acid. Characterization of the Nitrophenylphosphoric acid is done using various analytical methods.

Analytical Methods:

The characterization of Nitrophenylphosphoric acid is done using spectroscopic techniques such as Nuclear Magnetic Resonance (NMR) and Infrared (IR) Spectroscopy. Gas Chromatography Mass Spectrometry (GC-MS) and Liquid Chromatography Mass Spectrometry (LC-MS) are also used to analyze the compound.

Biological Properties:

Nitrophenylphosphoric acid has been found to have anti-cancer properties. A study was conducted which found that Nitrophenylphosphoric acid inhibited the growth of cancer cells in vitro. It was also found that Nitrophenylphosphoric acid had no toxicity towards normal cells.

Toxicity and Safety in Scientific Experiments:

Nitrophenylphosphoric acid is considered to be safe to use in scientific experiments. However, it is important to handle the compound with proper safety protocols. The compound should be handled in a fume hood, and appropriate personal protective equipment should be used.

Applications in Scientific Experiments:

Nitrophenylphosphoric acid is widely used in biological and chemical research. The compound is used as a reagent to detect the activity of phosphatases in biological samples. It is also used as an intermediate in the synthesis of various pharmaceuticals.

Current State of Research:

There is ongoing research on the use of Nitrophenylphosphoric acid in cancer treatment. Nitrophenylphosphoric acid is being investigated for its potential use as a chemotherapeutic agent.

Potential Implications in Various Fields of Research and Industry:

Nitrophenylphosphoric acid has potential applications in the pharmaceutical industry. It is used as an intermediate in the synthesis of various pharmaceuticals. The compound also shows promise in the development of new cancer therapies.

Limitations and Future Directions:

Despite the promising results of Nitrophenylphosphoric acid in cancer research, there are limitations to its use. One of the limitations is the potential toxicity of the compound towards normal cells. Future research should focus on finding ways to enhance the selectivity of Nitrophenylphosphoric acid towards cancer cells.

Future Directions:

1. Investigating the use of Nitrophenylphosphoric acid in combination with other chemotherapeutic agents.

2. Developing strategies to enhance the selectivity of Nitrophenylphosphoric acid towards cancer cells.

3. Investigating the potential use of Nitrophenylphosphoric acid in the treatment of other diseases.

4. Developing new analytical methods for the detection and quantification of Nitrophenylphosphoric acid in biological samples.

5. Investigating the potential use of Nitrophenylphosphoric acid in the development of new materials.

6. Studying the mechanism of action of Nitrophenylphosphoric acid in cancer cells.

7. Investigating the use of Nitrophenylphosphoric acid in the treatment of drug-resistant cancers.

8. Investigating the potential use of Nitrophenylphosphoric acid in agriculture as a pesticide.

9. Studying the pharmacokinetics and pharmacodynamics of Nitrophenylphosphoric acid in vivo.

10. Investigating the use of Nitrophenylphosphoric acid in the development of new imaging agents for cancer diagnosis.