6578-06-9 , BCIP p-toluidine,5-Bromo-4-chloro-3-indolyl phosphate p-toluidine-salt

6578-06-9 , BCIP p-toluidine,5-Bromo-4-chloro-3-indolyl phosphate p-toluidine-salt
C8H5BrClNO4P·C7H10N / 433.63
MFCD00040636

BCIP p-toluidine,5-Bromo-4-chloro-3-indolyl phosphate p-toluidine-salt

BCIP is a colorimetric substrate for alkaline phosphatase. Upon enzymatic cleavage by alkaline phosphatase, an indoxyl intermediate is released and subsequently oxidized to produce water-insoluble indigo. It can be used to detect alkaline phosphatase activity by immunohistochemistry (IHC) or Western blot (WB).

BCIP(BCIP p-toluidine salt; X-phosphate p-toluidine salt) is an artificial chromogenic substrate used for the sensitive colorimetric detection of alkaline phosphatase activity.

A substrate for visualising alkaline phosphatase activity. It produces a purple insoluble end product that is detected visually, when used together with Nitroblue tetrazolium. The substrate system is versatile and has functions in a variety of applications, including Northern, Southern, and Western blotting, in situ hybridization, ELISAs, microarray and immunohistochemistry. DMF soluble.

CAS Number	6578-06-9
Product Name	(5-bromo-4-chloro-1H-indol-3-yl) hydrogen phosphate;(4-methylphenyl)azanium
IUPAC Name	(5-bromo-4-chloro-1H-indol-3-yl) hydrogen phosphate;(4-methylphenyl)azanium
Molecular Formula	C₁₅H₁₅BrClN₂O₄P
Molecular Weight	433.62 g/mol
InChI	InChI=1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3
InChI Key	QEIFSLUFHRCVQL-UHFFFAOYSA-N
SMILES	CC1=CC=C(C=C1)[NH3+].C1=CC(=C(C2=C1NC=C2OP(=O)(O)[O-])Cl)Br
Canonical SMILES	CC1=CC=C(C=C1)[NH3+].C1=CC(=C(C2=C1NC=C2OP(=O)(O)[O-])Cl)Br