Bis(4-methylumbelliferyl)pyrophosphoric acid disodium salt;

4-Methylumbelliferyl pyrophosphate diester disodium salt

4-Methylumbelliferyl pyrophosphate diester disodium salt is a fluorescent compound that can be used as an enzyme substrate and a ligand. It is often used in diagnostics, environmental testing, food testing, and chemiluminescence assays. 4-Methylumbelliferone pyrophosphate diester disodium salt is a conjugate of the ligand 4-methylumbelliferyl phosphate with sodium pyrophosphate. This product has high purity and quality, and may be used for staining, bioluminescence and fluorescence detection of enzymes.

Bis(4-methylumbelliferyl)pyrophosphoric acid, disodium salt (BMPA-2Na) is an organic compound that is commonly used as a substrate for the detection and quantification of pyrophosphate. It has become increasingly popular in scientific research due to its diverse properties and applications. This paper will explore the definition, background, physical and chemical properties, synthesis, characterization, analytical methods, biological properties, toxicity and safety in scientific experiments, applications in scientific experiments, current state of research, potential implications in various fields of research and industry, limitations, and future directions of BMPA-2Na.

Definition and Background:

BMPA-2Na is a bis-analog of 4-methylumbelliferone and has the chemical formula C26H21Na2O13P2. It is a colorless to light yellow powder, which is soluble in water and has a molecular weight of 624.38 g/mol. Under the UV light, BMPA-2Na fluoresces, making it an ideal substrate for the detection and quantification of pyrophosphate.

Physical and Chemical Properties:

BMPA-2Na is a stable compound that is sensitive to moisture and light. It has a melting point of 242-245°C and a density of 1.55 g/cm3. BMPA-2Na can be easily hydrolyzed in the presence of water and phosphate ions to form 4-methylumbelliferyl phosphate. It is not soluble in organic solvents such as ethanol, methanol, chloroform, or acetone.

Synthesis and Characterization:

BMPA-2Na can be synthesized by the reaction of 4-methylumbelliferyl phosphate with phosphorus oxychloride followed by treatment with sodium hydroxide to form the disodium salt. The compound is characterized using various analytical methods such as NMR, IR, and MS.

Analytical Methods:

BMPA-2Na is used in various analytical methods such as fluorescence, spectrophotometry, and chromatography. The fluorescent properties of BMPA-2Na make it an ideal substrate for the detection and quantification of pyrophosphate in biological samples.

Biological Properties:

BMPA-2Na has been widely used in scientific studies as a substrate to measure pyrophosphate in biological samples. It has been reported to be non-toxic and non-carcinogenic in animal studies.

Toxicity and Safety in Scientific Experiments:

There is no evidence of BMPA-2Na being hazardous to human health. However, it is recommended to handle the compound using protective clothing and gloves as it can cause skin and eye irritation.

Applications in Scientific Experiments:

BMPA-2Na is widely used in scientific research for the detection and quantification of pyrophosphate in biological samples. It is used in various experiments such as enzyme assays, protein phosphatase assays, and nucleotide-binding assays.

Current State of Research:

Research on BMPA-2Na has been ongoing for several decades. Recent studies have focused on the development of new analytical techniques using BMPA-2Na as a substrate. Other studies have focused on the use of BMPA-2Na in drug discovery and development.

Potential Implications in Various Fields of Research and Industry:

The use of BMPA-2Na in scientific research has implications in various fields such as medicine, biochemistry, and molecular biology. The compound can be used in the development of new therapies for diseases such as cancer, Alzheimer's disease, and diabetes.

Limitations:

BMPA-2Na has some limitations such as its sensitivity to moisture and light. Additionally, the compound is sensitive to pH changes, which can affect its fluorescent properties.

Future Directions:

Future research can focus on the development of new analytical techniques that can use BMPA-2Na as a substrate. Studies can also focus on the use of BMPA-2Na in the development of new therapies for diseases. Other research can focus on the development of a more stable analog of BMPA-2Na with improved properties.