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1492-30-4 , 4-Nitrophenyl palmitate; 4-Nitrophenyl hexadecanoate; Palmitic acid 4-nitrophenyl ester

1492-30-4 , 4-Nitrophenyl palmitate;
4-Nitrophenyl hexadecanoate; Palmitic acid 4-nitrophenyl ester
C22H35NO4 / 377.52
MFCD00047732

4-Nitrophenyl palmitate

4-Nitrophenyl hexadecanoate; Palmitic acid 4-nitrophenyl ester


4-Nitrophenyl palmitate is a lipase-sensitive chromogenic substrate commonly used in the study of lipase and esterase activity. Its hydrolysis by lipases produces a yellow 4-nitrophenol, which can be easily detected spectrophotometrically, offering a quantitative measure of enzyme activity. This substrate is widely utilized in the screening of lipase-producing microorganisms and the optimization of enzyme-catalyzed reactions in various fields, including biotechnology and pharmaceutical research.

4-Nitrophenyl palmitate is a colorimetric lipase and esterase substrate. Upon enzymatic hydrolysis of 4-nitrophenyl palmitate, 4-nitrophenol is released which can be quantified by colorimetric detection at 410 nm as a measure of enzyme activity. 4-Nitrophenyl palmitate has been used to characterize the activity of various bacterial and mammalian enzymes, including Burkholderia and porcine pancreatic lipases.

P-nitrophenyl palmitate is a palmitate ester obtained by condensation of the carboxy group of palmitic acid with the phenolic hydroxy group of p-nitrophenol. It is a hexadecanoate ester and a C-nitro compound. It derives from a 4-nitrophenol.

CAS Number1492-30-4
Product Name4-Nitrophenyl palmitate
IUPAC Name(4-nitrophenyl) hexadecanoate
Molecular FormulaC22H35NO4
Molecular Weight377.5 g/mol
InChIInChI=1S/C22H35NO4/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-22(24)27-21-18-16-20(17-19-21)23(25)26/h16-19H,2-15H2,1H3
InChI KeyLVZSQWIWCANHPF-UHFFFAOYSA-N
SMILESCCCCCCCCCCCCCCCC(=O)OC1=CC=C(C=C1)[N+](=O)[O-]
Synonyms4-nitrophenyl palmitate, para-nitrophenyl palmitate
Canonical SMILESCCCCCCCCCCCCCCCC(=O)OC1=CC=C(C=C1)[N+](=O)[O-]


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