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7493-95-0 ,4-Nitrophenyl-α-D-galactopyranoside , CAS: 7493-95-0

7493-95-0 ,4-Nitrophenyl-α-D-galactopyranoside , CAS: 7493-95-0
C12H15NO8 / 301.25
MFCD00065050

4-Nitrophenyl a-D-galactopyranoside

The 4-Nitrophenyl-alpha-D-galactopyranoside is a high-quality chromogenic substrate specifically designed for the enzymatic assay of alpha-galactosidase. Upon enzyme catalysis, it yields a highly fluorescent 4-nitrophenol product, which allows easy and accurate measurement of enzyme activity in a variety of research applications, such as biochemical analysis, inhibitor screening, and enzyme kinetics studies.

CAS No: 7493-95-0 Synonyms: PNP-a-Gal MDL No: MFCD00065050 Chemical Formula: C12H15NO8 Molecular Weight: 301.25
CAS Number7493-95-0
Product Name4-nitrophenyl-alpha-D-galactopyranoside
IUPAC Name(2R,3R,4S,5R,6R)-2-(hydroxymethyl)-6-(4-nitrophenoxy)oxane-3,4,5-triol
Molecular FormulaC12H15NO8
Molecular Weight301.25 g/mol
InChIInChI=1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-3-1-6(2-4-7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12+/m1/s1
InChI KeyIFBHRQDFSNCLOZ-IIRVCBMXSA-N
SMILESC1=CC(=CC=C1[N+](=O)[O-])OC2C(C(C(C(O2)CO)O)O)O
Synonyms4-nitrophenyl-alpha-galactoside, 4-nitrophenylgalactoside, 4-nitrophenylgalactoside, (alpha-D)-isomer, p-nitrophenyl-alpha-D-galactopyranoside, p-nitrophenyl-alpha-galactoside, para-nitrophenyl-beta-D-galactoside, PNPG
Canonical SMILESC1=CC(=CC=C1[N+](=O)[O-])OC2C(C(C(C(O2)CO)O)O)O
Isomeric SMILESC1=CC(=CC=C1[N+](=O)[O-])O[C@@H]2[C@@H]([C@H]([C@H]([C@H](O2)CO)O)O)O

COA:

name:4-Nitrophenyl-α-D-galactopyranoside 

M.F.: C12H15O8          M.W.: 301.25                CAS: 7493-95-0

Items

Standards

Results

Appearance

White or slightly yellow crystal powder

Complies

Solubility

Soluble in DMF and insoluble in ether

Complies

NMR and MS

Should comply

Complies

Identification

IR and HPLC

Complies

Specific rotation

[α]20/D, (c=0.4, in water)

+218° ~ +222°

220°

Loss weight on dryness

Max. 1%

0.5%

Any impurity

Max. 0.5%

Complies

Residue on ignition

Max.1%

0.2%

Heavy metals

Max.20 ppm

Complies

TLC

Should be one spot

one spot

Purity

Min.98%

99.2%

Assay by HPLC

98% ~ 102%

99.4%


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