7493-95-0 ,4-Nitrophenyl-α-D-galactopyranoside , CAS: 7493-95-0

7493-95-0 ,4-Nitrophenyl-α-D-galactopyranoside , CAS: 7493-95-0
C12H15NO8 / 301.25
MFCD00065050

4-Nitrophenyl a-D-galactopyranoside

The 4-Nitrophenyl-alpha-D-galactopyranoside is a high-quality chromogenic substrate specifically designed for the enzymatic assay of alpha-galactosidase. Upon enzyme catalysis, it yields a highly fluorescent 4-nitrophenol product, which allows easy and accurate measurement of enzyme activity in a variety of research applications, such as biochemical analysis, inhibitor screening, and enzyme kinetics studies.

CAS No: 7493-95-0 Synonyms: PNP-a-Gal MDL No: MFCD00065050 Chemical Formula: C12H15NO8 Molecular Weight: 301.25

CAS Number	7493-95-0
Product Name	4-nitrophenyl-alpha-D-galactopyranoside
IUPAC Name	(2R,3R,4S,5R,6R)-2-(hydroxymethyl)-6-(4-nitrophenoxy)oxane-3,4,5-triol
Molecular Formula	C12H15NO8
Molecular Weight	301.25 g/mol
InChI	InChI=1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-3-1-6(2-4-7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12+/m1/s1
InChI Key	IFBHRQDFSNCLOZ-IIRVCBMXSA-N
SMILES	C1=CC(=CC=C1[N+](=O)[O-])OC2C(C(C(C(O2)CO)O)O)O
Synonyms	4-nitrophenyl-alpha-galactoside, 4-nitrophenylgalactoside, 4-nitrophenylgalactoside, (alpha-D)-isomer, p-nitrophenyl-alpha-D-galactopyranoside, p-nitrophenyl-alpha-galactoside, para-nitrophenyl-beta-D-galactoside, PNPG
Canonical SMILES	C1=CC(=CC=C1[N+](=O)[O-])OC2C(C(C(C(O2)CO)O)O)O
Isomeric SMILES	C1=CC(=CC=C1[N+](=O)[O-])O[C@@H]2[C@@H]([C@H]([C@H]([C@H](O2)CO)O)O)O

COA:

name:4-Nitrophenyl-α-D-galactopyranoside

M.F.: C12H15O8 M.W.: 301.25 CAS: 7493-95-0

*Items*	*Standards*	*Results*
Appearance	White or slightly yellow crystal powder	Complies
Solubility	Soluble in DMF and insoluble in ether	Complies
NMR and MS	Should comply	Complies
Identification	IR and HPLC	Complies
Specific rotation [α]20/D, (c=0.4, in water)	+218° ~ +222°	220°
Loss weight on dryness	Max. 1%	0.5%
Any impurity	Max. 0.5%	Complies
Residue on ignition	Max.1%	0.2%
Heavy metals	Max.20 ppm	Complies
TLC	Should be one spot	one spot
Purity	Min.98%	99.2%
Assay by HPLC	98% ~ 102%	99.4%