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117153-55-6 ,,4-Trifluoromethylumbelliferyl-b-D-galactopyranoside, CAS:117153-55-6

117153-55-6 ,
4-Trifluoromethylumbelliferyl-b-D-galactopyranoside,
CAS:117153-55-6
C16H15F3O8 / 392.28
MFCD00064056

4-Trifluoromethylumbelliferyl b-D-galactopyranoside

4-三氟甲基伞形酮-beta-D-半乳糖苷

4-Trifluoromethylumbelliferyl β-D-galactopyranoside is a chromogenic substrate that can be used to detect the presence of β-glucuronidase and β-galactosidase in a variety of organisms. This compound has been shown to be useful for the detection of bacteria such as E. coli, Salmonella, and Listeria monocytogenes. 4TFU has been shown to hydrolyze at pH values between 5 and 8. The reaction starts with a color change from yellow to blue at pH 5, which changes again to green at pH 8. The equivalence point for this substrate is at pH 6.4, where it produces an orange color.

Blue-green fluorogenic probe with higher fluorescence in the physiological pH range due to lower pKa. Suitable for the detection of LacZ without the need of a stop buffer treatment step for development of fluorescence.

CAS Number117153-55-6
Product Name4-(Trifluoromethyl)-7-[(2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxychromen-2-one
IUPAC Name4-(trifluoromethyl)-7-[(2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxychromen-2-one
Molecular FormulaC16H15F3O8
Molecular Weight392.28 g/mol
InChIInChI=1S/C16H15F3O8/c17-16(18,19)8-4-11(21)26-9-3-6(1-2-7(8)9)25-15-14(24)13(23)12(22)10(5-20)27-15/h1-4,10,12-15,20,22-24H,5H2/t10-,12+,13+,14-,15-/m1/s1
InChI KeyWXZJYRMHQCZVKG-BGNCJLHMSA-N
SMILESC1=CC2=C(C=C1OC3C(C(C(C(O3)CO)O)O)O)OC(=O)C=C2C(F)(F)F
Synonyms4-trifluoromethylumbelliferyl-beta-D-galactopyranoside, 4-trifluoromethylumbelliferylgalactopyranoside, TFMU-gal
Canonical SMILESC1=CC2=C(C=C1OC3C(C(C(C(O3)CO)O)O)O)OC(=O)C=C2C(F)(F)F
Isomeric SMILESC1=CC2=C(C=C1O[C@H]3[C@@H]([C@H]([C@H]([C@H](O3)CO)O)O)O)OC(=O)C=C2C(F)(F)F
CAS No: 117153-55-6 Synonyms: TFMU-b-Gal MDL No: MFCD00064056 Chemical Formula: C16H15F3O8 Molecular Weight: 392.28
References: 1. Karpova EA, Voznyi YAV, Dudukina TV, Tsvetkova IV,, Biochem. Int., 1991, 24, p1136


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